On RFLPs, chromosome walking, etc!
posted by pam39 at 11:23 AM
Thursday, July 12, 2007
BIOL335 links
- Your textbook!
- UBC BIOL335 website(updated)
- More than you'll need to know on P elements
- Link to a site on Ac/Ds elements
- Landmark discoveries in molecular biology
- Link to mol.gen. of prokaryotes course at univ. of Arizona
- Lac operon stuff
- Link to a clatech problem set
- Link to another caltech problem set
- Link to Dr. Zimmerman's molbiol-check out their links!
- BIOL334 website
Genetics links
- Your textbook!
- Traditional genetics terms
- Human genetics problem sets (University of Arizona)
- Mixed problem set
- Another problem set (vcu)
- Another problem set (vcu)
- Pam's loppins questions
- Triploid bananas: how does it work?
- BIOL334 website
Previous Posts
- More questions
- markers question
- extra help session
- post-midterm thoughts
- A few answers
- QUESTIONS THAT CAME UP!
- TECHNIQUUES
- ANNOUNCEMENT
- SOME QUESTIONS THAT CAME UP
- WELCOME TO BIOL335!
16 Comments:
Hey Pam,
Craig told us to catch up on reading and start studying chapter 17 as well for Friday, but he also mentioned that we would hardly be covering any of chapter 17 and mainly focusing on 18 and the earlier chapters. Is there any particular sections or pages of chapter 17 we should read?
what about VNTR's?
For chapter 17, tables 17-1 and 17-2 are good, plus all the class notes you'll have.
I don't know...what about them (VNTRs)?
Pam
how do you use VNTR's to do a cloning project, is it the same way as it is with RFLP and chromosome walking?
Pam, major question
what's the difference between SNP and RFLP? RFLP are where restriction sites are used to determine morphs within a family/population and to determine the location of the diseased gene of interest
SNP, on the other hand are just changes in a single nucleotide, so that means that the restriction enzyme recognition site is disrupted therefore we lose an RFLP site???
can we have some practice chromosome walking q's please
Pam, do P-elements have IR (inverted repeats) on either ends like IS elements have?
those IR regions are responsile of catching and using the transposase, made by the helper P-element, and allowing the gene to jump into the DNA or e.coli right?
Pam, on the sheets you posted above on chromosome walking, the second page where we are looking for the gene of interest by comparing it healthy individuals. We probe the healthy individuals' genomic library with the same probe as was used to find an open reading frame in the diseased population and if we can't find an ORF there then we use another clone. What is the other clone, is it just what the probe hybridizes to in the healthy individuals and we take the biggest piece since there are no ORF's or promotors found and that will be our new probe to find the an ORF and ultimately our gene when we compare this region to the diseased???
Good to see some comments...
P elements have "P feet", the equivalent of IRs.
Yes, some SNPs result in the formation or disruption of RFLPs.
About the chromosome walking "other clone"-yes, you are correct.
Cheers
Pam
Pam, can you please make up some more questions that could possibly be on the exam for us to work on together in class please please please
I was wondering since our avg. was really low, if we do better on the final exam, he'll just count better exam mark instead..???
OMG, the midterm was horrible, I got zero on one of the questions! So i really hope he does give us a deal
In every course I have taken so far, whenever there has been a hard midterm there has always been an easy final, and often vice versa. I'm only hoping this same pattern follows.
Does anyone know what the average was?
50% was the avg!
seriously, do you think he'll make it any easier? I honestly thought that the midterm was going to be hit out of the ball park, but it wasn't, I have little to no faith left as far as genetics is concerned. If majority of the class failed then there is a problem there and he should give us some type of break! Well, I hope we all pass the final cuz I don't want to do this again :(
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