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posted by pam39 at 4:54 PM
Wednesday, July 11, 2007
BIOL335 links
- Your textbook!
- UBC BIOL335 website(updated)
- More than you'll need to know on P elements
- Link to a site on Ac/Ds elements
- Landmark discoveries in molecular biology
- Link to mol.gen. of prokaryotes course at univ. of Arizona
- Lac operon stuff
- Link to a clatech problem set
- Link to another caltech problem set
- Link to Dr. Zimmerman's molbiol-check out their links!
- BIOL334 website
Genetics links
- Your textbook!
- Traditional genetics terms
- Human genetics problem sets (University of Arizona)
- Mixed problem set
- Another problem set (vcu)
- Another problem set (vcu)
- Pam's loppins questions
- Triploid bananas: how does it work?
- BIOL334 website
Previous Posts
- markers question
- extra help session
- post-midterm thoughts
- A few answers
- QUESTIONS THAT CAME UP!
- TECHNIQUUES
- ANNOUNCEMENT
- SOME QUESTIONS THAT CAME UP
- WELCOME TO BIOL335!
- Review and quant genetics
14 Comments:
For the bottom q about the cooking
are the RFLP loci different for each family?
I have no idea where to begin with the first q
For the cooking question we are looking at the same RFLP in everybody's DNA (exactly the same locus. The 2 families are not related.
For the first question, step #1=reading it carefully, step2=writing aout the crosses, assigning possible genotypes, write down expected progenies, compare to observed progenies...and if there is a mismatch, explaining it!
Cheers
Pam
so for 2, it's the same RFLP for noth families but it's the probe that differs? That is, the disease from one parent is on one probe and the disease in the other parent is from the other probe?
Same probe, same restriction enzyme used for EVERYONE.
Think carefully about what a Southern blot is, and what an RFLP is.
See you in class!
Pam
Hi Pam, I was wondering what exactly the process is of chromosome walking and how it can be used to clone the gene of interest? Craig keeps asking us how would you clone the gene when you have found the proper RFLP and I have no idea. Can you maybe send us a link or something to a step by step process? Thanks
Hi, Pam. Could we go over yesterday's pedigree question in tutorial today?
Hi, Pam. Could we go over yesterday's pedigree question in tutorial today?
For VNTR question that we did in tutorial, We have two VNTRs(VNTR A: 1,5 & VNTR B: 2,3,4) and 5 alleles right? Also there are two loci?
I don't understand the VNTR pedigree, can someone please explain it to me :)
Pam, are we going to go over the questions in the tutorial?
Pam, I was wondering if you can understand my logic of explaining Loppins question makes sense.
e+ = eyelash e-= no eyelash eTE = no eyelash (transposable element inserted within eyelash gene
1. First cross that made between male with no eyelash x female eyelash produced all eyelash progenies in F1. This shows that eyelash is dominant over no eyelash. Selfing F1 produced perfect 3:1 mendelian ratio (3 eyelash : 1 no eyelash) shows that again eyelash is Wild type. Also, 3:1 shows typical monohybrid ratio (not a sex linkage)
2. 2nd cross made between male with no eyelash (from 1st cross) x female with no eyelash (found under car) produces no eyelash progeny with some progeny having half eyelash/half no eyelash due to transposable element in effect.
3. If this was in traditional mendelian ratio, you'd expect all no eyelash right? Since it's a cross between no eyelash.
4. male no eyelash (from 1st cross) x female no eyelash (found under car)
P: e-/e- (male) x e-/eTE (female)
F1: most e-/e- but some half no eyelash/half eyelash
When the male (e-/e-) and female (e-/eTE) mated, some of the gametes have received repressors from mother so P-element is prevented from jumping. Therefore, some of progeny shows half eyelash/half no eyelashes.
I know this is really long.. But I want to be sure if my logic is correct.
P.S. P-element is only presented in germline right?? It can't be presented in somatic cell right?
Oooh, I have question about GPCR!
Why do we need liposomes??
We emerge insect cells with liposomes containing plasmid & antibiotic resistance marker right?
So basically we want liposomes to integrate into insect nucleus???
I'm confused. -_-
Pam, do we have to know about CDKs?? Craig only went over GPCRs..
I'm just worried that he told us to focus more on lecture and he tests on something weird on final...
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